PJB-2019-690
DUPLICATED B-CLASS AND C-CLASS MADS-BOX GENES REVEAL DISTINCT ROLES IN BASAL EUDICOTS EPIMEDIUM SAGITTATUM
Ming Li
Abstract
Flowers of the basal eudicot Epimedium (Barrenwort) possess morphological innovations, namely petal spurs with nectar and petaloid sepals. The floral homeotic B function gene APETALAS3 (AP3) and PISTILLA (PI) determine sepal and petal identity and C function gene AGAMOUS (AG) confers stamen and carpel identity in Arabidopsis. Complex histories of gene duplication resulted in subsequent subfunctionalization or neofunctionalization of paralogs. In this study, we report the isolation of four B- and two C-class genes from E. sagittatum. Phylogenetic analysis showed that EsAP3-1, EsAP3-2 and EsAP3-3 belong to the AP3 clade; EsPI belongs to PI group; EsAG and EsAG11 clustered into AG and AG11 groups respectively. Quantitative real-time PCR detect their expression pattern, all B-class genes but EsAP3-3 universally expressed in floral organs. The expression of EsAG and EsAG11 genes were restricted to reproductive organs. In addition, yeast two and three hybrids to explore status of protein complex. EsAP3-1 protein has broadly interactive partners, EsPI protein can form a heterodimer with EsAP3-1 and EsAP3-2. Transgenic experiment of EsAG overexpression in wild-type Arabidopsis confirmed the conserved function determining carpel development.
To Cite this article:
Download
