PJB-2019-713
DETECTION OF TULIP BREAKING VIRUS BY RT-PCR
Rongchun Ye
Abstract
Tulip breaking virus (TBV) is the most devastating virus found in tulips. The virus is not only reducing quality of tulip cut flower but also decreasing yield of its bulbs. Visual inspection is time and labor consuming in large scale growing. Besides, it is difficult to identify TBV infected plants in cultivars with yellow, white, and dark purple flowers. This study is aimed at establishment of a fast and effective method for TBV detection using tulip leaves. Two specific primer combinations were designed according to amino acid sequences of polypeptide protein and coat protein of TBV. Reverse transcription-polymerase chain reaction (RT-PCR) detection system was established for TBV identification and the effectiveness of the system was verified in different tulip cultivars. Results showed that the primers have good specificity and the sequences of amplified product presented 99%-93% identity with reported TBV sequences. Amplification of TBV sequences in different tulip cultivars confirmed that the RT-PCR system for TBV detection is stable and reliable. The primers could be widely used for TBV detection in tulip plants. The results of this study could greatly facilitate fast detection of TBV in tulip plants and epidemiological studies of this important pathogen.
To Cite this article:
Download
