PJB-2019-741
Analyses of In-vitro antioxidant and anti-cancer activity of Cissus quadrangularis stem extract in osteoblastic cell line -UMR- 106.
Dr. Suchitra M. R
Abstract
Background: Cancer is a multistep disease andis one of the leading causes of death worldwide. In India it is estimated that 7.8 lakh cases of death occurred due to cancer in 2018 .Less than 1% is related to bone cancer. Osteosarcoma, a primary tumour of the bone is the second highest cause of bone cancer related death in young people. It is treated with chemotherapy, radiation therapy, and surgery whichcauseside effects. We hypothesized that certain traditional plants ,may have antitumour activities. This research aimed to evaluate the invitroantioxidant and apoptosis induced activity of stem extract of cissus quadrangularis (CQ) on osteoblastic cell line (UMR-106). Methodology: Various extracts of CQ was subjected to phytochemical screening and antioxidant studies. Ethanolic extract of CQ showed maximum phytoconstituents and better antioxidant activity for both DPPH(2,2-diphenyl-1-picryl-hydrazyl-hydrate)and FRAP(Ferric reducing antioxidant potential) assay.The UMR-106 cell line was treated with different concentrations of the extract ranging from 25μg to 400μg for varying time periods. The dose and time optimization of theethanolic extract was evaluated in the osteoblastic cell line (UMR-106) using MTT assay(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)which showed IC50 value with 200μg/ml at 24 hours. Results: There was a reduction in the percentage of cells by 50% in the treated group by SRB assay showed the cytotoxic potency of the extract. Flow cytometry analysis indicated that the extract of CQ induced apoptosis in UMR-106 cell line. The present study suggests that the stem extract of CQ possess significant antioxidant and anticancer activity in osteoblastic cell line and induces apoptosis in a dose dependent manner. Conclusion:Ethanolic extract of CQ induces G0/G1 arrest and apoptosis in osteoblasts, suggesting that this might represent a novel and effective agent against bone cancer.
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