PJB-2019-743
Cloning and characterization of two gibberellin receptor genes in yam (Dioscorea opposita)
Wen-Hong Long, Sheng-Sheng Xu, Jin-Gui Meng, Jun-Xia Yang, Rong-Ping Yang, Yan-Bi Duan, Xue-Mei Zhang and Chun-Hua Ma
Abstract
GID1 (gibberellin insensitive dwarf1) is a gibberellin receptor and plays an important role in the function of gibberellin. The full-length cDNA sequences of gibberellin receptor genes DoGID1B (GenBank No. MK253679) and DoGID1B2 (GenBank No. MK301538) were cloned by RT-PCR and RACE from yam (Dioscorea opposita). The expression of genes DoGID1B and DoGID1B2 in yam bulbils in dormant and sprouting stage and its response to paclobutrazol were studied by RT-qPCR. Amino acid composition, physicochemical properties, hydrophilicity, signal peptide, transmembrane structure, curled spiral composition and domain of the two deduced protein DoGID1B and DoGID1B2 were highly similar to GID1 proteins of Arabidopsis thaliana (GenBank No. NM_116166.5) and rice (Oryza sativa Japonica, GenBank No. XM_015784475.2). Both DoGID1B and DoGID1B2 proteins contain binding sites for hormones GA, repressor protein DELLA and hormone-sensitive lipase. Multiple sequence alignment indicated that the deduced DoGID1B and DoGID1B2 proteins shared high sequence identity with GID1s from other species. Phylogenetic analysis showed that DoGID1B and DoGID1B2 proteins were grouped in the clade of monocotyledon GID1, but the two proteins were clustered in different sub-clades. RT-qPCR studies showed that the expression of DoGID1B and DoGID1B2 genes was up-regulated when yam bulbils sprouting, and the up-regulation of DoGID1B was higher than that of DoGID1B2. In addition, the up-regulation could be shifted earlier by paclobutrazol. These findings help us to reveal the molecular mechanism of gibberellin transduction and to regulate the growth and sprouting of yam
To Cite this article:
Long, W.H., S.S. Xu, J.G. Meng, J.X. Yang, R.P. Yang, Y.B. Duan, X.M. Zhang and C.H. Ma. 2021. Cloning and characterization of two gibberellin receptor genes in yam (Dioscorea opposita). Pak. J. Bot., 53(5): DOI: http://dx.doi.org/10.30848/PJB2021-5(37)
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