PJB-2019-750
Effects of Cobalt Ions and Cobalt Nanoparticles on Transient Expression of gus Gene in Catharanthus roseus Suspension Cultures.
Ahmed Sayed Fouad
Abstract
Catharanthus roseus suspension cultures were inoculated with Agrobacterium tumefaciens strain carrying β-glucuronidase (gus) gene in presence of either Co2+ or cobalt nanoparticles (CoNPs) at 2.5, 5, 7.5 and 10 mg/L. Co2+ at 2.5 mg/L as well as CoNPs at 2.5, 5 and 7.5 mg/L enhanced expression of gus gene in plant cells. The enhancing effects can be attributed to the observed increase in virulence B1 (virB1) gene expression and potential subsequent improvement in transformation efficiency through the inhibitory effect of cobalt on ethylene biosynthesis in plant cells. Ethylene is a major hindrance against transformation success through suppression of vir genes expression. The Agrobacterium-friend role of cobalt was lost upon further increase in cobalt concentration due to abusing defense genes working against both heavy metal-induced oxidative stress and Agrobacterium-associated biotic stress. Results reflected better performance of CoNPs, compared with Co2+ in enhancement of expression of gus gene that can be attributed to lower sensitivity of plant defense system to cobalt nano form than ionic form. Such enhancements may suggest a potential role for CoNPs in improving Agrobacterium-mediated transformation that needs further investigation.
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