PJB-2019-766
GLUTENIN COMPOSTION OF WHEAT LANRACES COLLECTED FROM REMOTE AREAS WITH IMPROVED LINES OF KHYBER PAKHTUNKHUWA, PAKISTAN
S.F. Wadood
Abstract
ABSTRACT To explore variation in wheat landraces of indigenous wheat and commercially grown improved genotypes regarding high molecular weight glutenin constitution, 62 landraces and 10 improved wheat cultivars were collected from hilly northern areas of Pakistan to check their composition of HMW-Glutenin using the biochemical technique: SDS-PAGE. There were 7 alleles found for the HMW-GS at loci G-A1, G-B1 and G-D1 subsequently resulted in about four HMW-GS distinctive combinations. The combinations were, however, observed null at loci “6+8 and 2+12” appeared as dominant phenotype in about 62.50% of the total collected wheat genotypes. The ranking of these genotypes based on the quality score were ranged from 4 to 9. The highest quality score (9) was observed in about 13.86% advance cultivars at 7+9, 5+10 loci. The dendrogram constructed based on genetic resemblance background of HMW-GS split the genotypes into clusters A and B; which further bifurcated into three sub-clusters. The genotypes with less diversification revealed 100% similarity and resilient genetic linkage. This might be due to sharing of the same gene pool but also exposed loss of the genetic variability in wheat crop in Pakistan. To discourage such a genetic loss, it is imperative to analyze the wheat lines for quality assurance, genetic diversification and to make more strengthened future breeding strategies for sustainable cultivation of wheat crop in Pakistan.
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