PJB-2019-786
GENOME SIZE ESTIMATION OF POLYGONATUM KINGIANUM BY FLOW CYTOMETRY
PENG-ZHANG JI
Abstract
Flow cytometry was used to estimate the genome size of Polygonatum kingianum, a well-known traditional Chinese herb. The genomic size was reported for the first time in this study. The results showed the genome size of P. kingianum to be approximately 6.90±0.41 (pg). The HG3 had largest genome size of 7.28±0.38 (pg). Otherwise, the HG2 had the least genome size of 6.46±0.27 (pg), and the genome size of HG1 was 6.95±0.26 (pg). The difference of genome size was observed among three groups. The difference was 12.69% between HG2 and HG3. Whereas, the variation of 7.59% for HG1 and HG2, 4.75% for HG1 and HG3. It was related to the species diversity, environmental differences and planting patterns possibly. These results provide a foundation on the study of genome sequencing and genetic evolution of P. kingianum in the future.
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