PJB-2019-791
Influence of flooding root system of Lupinus termis and its impact on metabolites, metabolic enzymes and antioxidant system
Hamed M El-Shora1
Abstract
The loss of crop yield by flooding influences global food production. In order to tolerate hypoxic stress, plants undergo metabolic and biochemical changes. When Lupinus termis L was exposed to flooding the activity of nitrite reductase was retarded however, those of pyruvate decarboxylase (PDC), glutamate decarboxylase (GDC) and arginine decarboxylase (ADC) were induced. Lactate, nitrite and ethanol were enhanced whereas ATP production was inhibited. The activities of alcohol dehydrogenase (ADH), lactate dehydrogenase (LDH), malic enzyme (ME), glutamine synthetase (GS), glutamate synthase (GOGAT), alanine transaminase (ALAT) and nitrate reductase (NR) were induced. Malondialdehyde (MDA) and H2O2 contents increased indicating fatty acid oxidation in the presence of free radicals generated by flooding. The plant exhibited positive abilities in their antioxidant systems including superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR) and glutathione peroxidase (GP), in addition to ascorbate and reduced glutathione as non-enzymatic antioxidants. The positive antioxidant response might be responsible for a higher tolerance to flooding stress. The results reveal that accumulation of ethanol and lactate enable regeneration of reduced coenzymes and generation of ATP under flooding stress. Furthermore, production of succinate could confer tolerance, through production of ATP and serve as a mean of regulating the cellular redox potential.
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