PJB-2019-839
NITROGEN FERTILIZATION IMPROVES BIOMASS YIELD AND SELECTED BIOACTIVE CONSTITUENTS OF ORTHOSIPHON STAMINEUS LEAF
Noorhaslina Binti Hashim
Abstract
Generally, sufficient supplied of macronutrient in plants is most recommended for plant growth. Furthermore, the aim of this study to investigate the effects of nitrogen deficiency on biomass yield and bioactive compound concentration of Orthosiphon stamineus leaf. The experiment consist of four rate nitrogen fertilization [0 N kg/ha (high N stress), 110 N kg/ha (medium N stress), 170 N kg/ha (low N stress) and 250 N kg/ha (Control)]. The nitrogen fertilization was split into three times fertilization (4 weeks after sowing, week 4 and week 6) and each time was applied at 33.3% of total nitrogen fertilization. The findings of this study clearly show that the O. stamineus leaf grown under nitrogen stress significantly affected the rosmarinic acid (RA), 3′-hydroxy-5, 6, 7, 4 ′-tetramethoxyflavone (TMF), sinensetin (SEN) and Eupatorin (EUP) concentration. It was also found that nitrogen fertilization most affected the biomass yield of the O. stamineus leaf in medium and control N stress levels. One explanation of this study indicated that the nitrogen supply for medium stress level was upsurge the yield of biomass production and total bioactive compounds for O. stamineus leaf.
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