PJB-2019-895
Induced tetraploid increases drought-tolerance in Actinidia chinensis
Xianang Zhang
Abstract
Drought is an important factor that restricts plant growth, development, and yield of Actinidia chinensis. To breed drought-resistant varieties and study whether the expression of drought-resistant substances in polyploid plantlets of A. chinensis has a dose effect, ‘SWFU02’ and its tetraploid plantlets were used as experimental materials, and polyethylene glycol (PEG)-6000 was used to induce drought stress in A. chinensis, and the related physiological indices were determined, and the expression of drought-resistance-related genes was analysed by qPCR. The results showed that the tetraploid plantlets had better drought resistance than diploid ones, and the contents of TRE, SPC, and PRO were significantly higher in tetraploidy than those in diploidy, however, the activities of CAT and SOD were significantly decreased. The expression of DLD2 and TPS1 genes in tetraploidy increased significantly and the expression level of MIOX gene was significantly down-regulated compared to diploidy. The dose effect of the drought-resistant related substances like PRO, SPC, and TRE in polyploid plantlets of A. chinensis was obvious. And 210 drought-resistant polyploid plantlets of A. chinensis were bred.
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