PJB-2019-942
Investigation of DNA methylation level in wheat genome exposed to vanadium by using CRED-RA technique
Ozlem Bakir
Abstract
Vanadium (V) is one of the potential ecological hazards due to anthropogenic activities. Most important anthropogenic V sources; fossil fuels, the combustion of coal or petroleum products, the contamination from the use of fertilizers and alloys in the steel industry. V has become a serious pollutant due to its widespread use in the industry. In this study, DNA methylation changes in Triticum aestivum L. ‘Çetinel 2000’ seeds were investigated with the increasing doses (4.4, 6.6, 8.8 mM) of V and two different doses of boric acid (BA) (4 and 8 mM) by CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) analyses. DNA band changes were demonstrated by InterSimple Sequence Repeats (ISSR) analysis. According to CRED-RA analysis, it was seen that V application caused more changes in DNA methylation level in wheat genome than BA application. Because little is known about significant molecular mechanisms related to V toxicity and resistance / tolerance, the CRED-RA technique can be used to detect changes in DNA methylation levels in plants exposed to abiotic stress conditions such as heavy metal. As a result of CRED-RA analyzes, high polymorphism and methylation model changes were observed.
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