PJB-2005-118
INDUCTION OF SYSTEMIC RESISTANCE IN CHICKPEA AGAINST FUSARIUM WILT BY SEED TREATMENT WITH SALICYLIC ACID AND BION
NIGHAT SARWAR, M. HAYAT ZAHID CH., IKRAMUL HAQ, AND F. F. JAMIL
Abstract
Seeds of chickpea variety AUG424, susceptible to Fusarium oxysporum ciceri (FOC), were surface sterilized with sodium hypochlorite, thoroughly rinsed with sterilized water and immersed in two concentrations of Salicylic acid (1.0 & 1.5 mM) and Bion (0.3& 0.4mM). Seeds treated with 2% Benlate were used as standard. Control represented, the seeds soaked in distilled water. Control and treated seeds were sown in two sets of pots containing sterilized soil (experiment 1) and soil inoculated with FOC (experiment 2). Chemically treated and control seeds were grown under controlled environment. Two week old seedlings grown in sterilized soil were up rooted, roots were cut at 1cm from tip and immersed in spore suspension (106 micro conidia/ml) of FOC for three hours and then the seedlings were transplanted into new pots containing sterilized soil. Plants of both sets were observed daily for up to 40 days to record wilt disease by counting the total and wilted plants in each pot. At the end of the experiment, surviving plants were cut at collar region for observing the fungus growth inside the vascular tissues. Fresh and dry weight of the shoots and roots were recorded. Wilt disease was significantly reduced with all the treatments in both experiments. On the basis of disease rating done after root cutting, wilt incidence was significantly less in chemically treated plants as compared to control ones. Fresh and dry weights of shoot and root were higher in treated plants as compared to control ones especially in plants grown from Bion treated seeds.
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