PJB-2019-957
BIOCHEMICAL AND METABOLIC CHARACTERIZATION OF SOIL MICROFLORA FROM VEGETABLE FARM
AISHA AZHAR
Abstract
Soil microorganisms particularly plant growth promoting rhizobacteria (PGPR) are basic to uphold the sustainability of agricultural land. In the present research, samples of rhizospheric soil were collected from vegetable farm, University of the Punjab, Lahore. Eleven strains of bacteria were isolated, purified and characterized biochemically and metabolically by performing different tests such as nitrogen fixation, phosphate solublization, synthesis of indole acetic acid (IAA), hydrogen cyanide (HCN) and H2S production, susceptibility to antibiotics and heavy metals. Most of the bacterial strains exhibited positive result for different tests and characterized as Staphylococcus aureus, Staphylococcus saprophyticus and Micrococcus varians. As most of the strains were positive for nitrogen fixation, IAA production, phosphate solublization and resistance against heavy metals; in this way, these strains may well be utilized for removal of substantial metals from the ecosystem and also as biofertilizer to improve plant yield and efficiency in vegetable farm.
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