PJB-2019-972
MUTAGEN EFFECT ON GROWTH, PHYSIOLOGY AND MICROSTRUCTURE OF ALFALFA
SHEN XIAOHUI
Abstract
In order to study the effect of mutagenesis on growth, physiology and cell structure in alfalfa (Medicago sativa L), the seeds of a local variety ‘Longmu806’ were treated in the test with two kinds of mutagenesis methods, including 60Co-γ irradiation (50, 150, 300, 450, 600 Gy) and EMS (ethyl methane sulfonate 0.1%, 0.2%, 0.4% (v/v)). The result showed that a significant increase in seed germination percentage and seedling growth began from 50 to 150 Gy of 60Co-γ irradiation and then decreased with increasing radiation doses. However, the germination percentage and seedling growth significant decreased by EMS treated as compared to the control. The single plant fresh grass production and plant height of the two mutagenesis treatments was similar to that of germination rate. A significant enhancement of branch number was observed in response to increasing 60Co-γ irradiation dose and EMS concentrations. The chlorophyll content was promoted from 50 to 150Gy dose and 0.4% EMS concentration. It was also found that mutagenic treatments could promote the activity of three anti-oxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and hydrogen peroxidase (CAT). Under stress, the leaf thicknesses were increased, the vein protuberant degrees was decreased and the compactness of cell structure was enhanced. Being more conducive for screening mutants, 60Co-γ irradiation with 150Gy and EMS with 0.4% were the ideal mutagenic treatments. This study provides an insight into the potential use of mutation in manipulating growth parameters of alfalfa and enhances understanding of the physiological responses inflicted by irradiation and EMS stress.
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