PJB-2019-974
POTOCOLS OPTIMIZATION FOR IN VITRO PROPAGATION OF JATROPHA CURCAS
MUHAMMAD QASIM
Abstract
Jatropha curcas is a tremendous source of biodiesel with seeds containing 30-40% oil content. The plant also exhibit diverse medicinal values, but Jatropha production is negligible due to fluctuations in weather causing seed dormancy. The micropropagation of this economically important plant is the basic requirement of present era to cope its high demands for biodiesel production. Applications of various phytohormones significantly increase the micropropagation of Jatropha. The present study revealed that MS added with BAP (1.0 mg/l) germinated in shortest possible time (10.18) and shoot induction (10.60), while MS media added with IBA (1.0 mg/l) presented less days to root initiation (10.64). The maximum germination percentage (73.49%) was recorded on media containing BAP (1.0 mg/l), while less bacterial contamination (1.61%) was seen in MS plain media. MS media added with with GA3 (1.0 mg/l) geminated in very less time in seeds of Jatropha curcas. The highest no. of shoots (80) were seen in MS add on with BAP (1.0 mg/l) in seedlings. The MS media having 2, 4-D + BAP (0.5 mg/l), respectively, depicted maximum production of leaves-derived callus initiation (100%). Then the plantlets were subjected to acclimatization and subsequently transferred to soil that reached to maturity and produced normal seeds. The optimized protocols for micropropagation would be beneficial for the mass production of economically important Jatropha plant.
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