PJB-2019-983
Phytochemical Analysis and Antibacterial Activity of Tannins Extracted from Salix alba L. Against Different Gram-Positive and Gram-Negative Bacterial Strains
Bilal Javed
Abstract
Special ability of tannins to precipice proteins, prevent the availability of substrate to bacterial cells, deprivation of extracellular microbial enzyme, direct action on microbial mechanism and iron deprivation make them a suitable candidate to control the proliferation of resistant bacterial strains. Antibacterial activity of tannins extracted from Salix alba leaves and stem bark methanolic extracts were determined by using agar well diffusion assay against seven resistant gram-positive and gram-negative bacterial strains including Streptococcus pyrogenes, Staphylococcus aureus (1), Staphylococcus aureus (2), Shigella sonnei, Escherichia coli (1), Escherichia coli (2) and Neisseria gonorrhoeae and zone of inhibition was measured in term of millimeter. It was observed that the tannins that are extracted from the bark of the S. alba are more effective to kill all bacterial pathogens and the inhibition percentage increased with increasing the concentration of the extracted tannins. Photochemical analysis was carried out in term of qualitative and quantitative phytochemical screening for major classes of secondary metabolites e.g. Alkaloids, Flavonoids, Oils/Fats, Saponins, Tannins, Quinones, Steroids, Resins and Phenols. Leaves fraction of Salix alba L. was enriched with treasury of Tannins, Quinones, Steroids, Resins and Phenols while stem bark extracts were embellished with Flavonoids, Tannins, Quinones and Phenols. Findings from this study will help to develop formulation from tannins extracted from S. alba and endorse the ethnopharmacological use of the evaluated plant.
To Cite this article:
Download
