PJB-2019-992
DETECTION OF ABSCISIC ACID (ABA) AND RELATIVE TRANSCRIPT ABUNDANCE OF ABA-RELATED GENES IN Syntrichia caninervis
Yigong Zhang
Abstract
Abscisic acid (ABA) is a plant hormone known to regulate environmental stress responses in various species. In bryophytes, only a limited number of species have been reported to have endogenous ABA. In Pottiaceae, Syntrichia ruralis ABA was not been detected. However, in Syntrichia caninervis, we used Ultra Performance Liquid Chromatography-mass spectrometry (UPLC-MS) to determine the content of endogenous ABA in gametophores. Gametophores contained 7.96–30.87 ng/g dry weight of ABA during dehydration. qRT-PCR analysis was used to examine transcript abundance of ABA-related genes (ZEP, NCED3, SnRK2, ABI1, and ABI5) during dehydration, rehydration, ABA treatments, and cold stress. The data indicates that two similar species, S. caninervis and S. ruralis, have distinctly different amounts of ABA in drying gametophores. The study brings a new insight into understanding the physiological roles as well as the biosynthetic, metabolic, and signaling pathways of ABA in bryophytes.
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