PJB-2019-995
EFFECT OF PLANT GROWTH REGULATORS AND EXPLANT ON REGENERATION AND SHOOT DEVELOPMENT OF SOYBEAN (GLYCINE MAX CV. SAMAN)
Vahid Mehrizadeh
Abstract
Soybean is one of the most important sources of oil and protein around the world. Classical breeding programs for soybean are limited because of its self-pollination ability. Genetic transformation of soybean is necessary for improvement of the variety. Developing an efficient plant regeneration and genetic transformation protocol is the first step in soybean transformation programs. Saman is an important commercial soybean cultivar, being used in Iran. In this study effect of some important factors, including explant (cotyledon, hypocotyl and leaf) and various combinations of plant growth regulators (BAP, KIN, IAA and IBA) on seed germination and plant regeneration of soybean were studied. The results showed that the maximum percentages of germination of soybean seeds were obtained on MS medium supplemented with 2 mg/l BAP. The highest number of shoot induction was obtained in the presence of 1.5 mg/l BAP and 0.1 mg/l IAA. Also, the cotyledon explants were far better than other explants. The maximum shoot elongation and development was obtained on MS medium containing 0.2 mg/l BAP and 0.5 mg/l GA3. Rooting of elongated shoots was achieved on half strength MS medium augmented with 1 mg/l IBA. The plantlets regenerated in vitro with well-developed shoot and roots was successfully established in pots containing garden soil and grown in a greenhouse with a high survival rate. In the present study, we found inclusion BAP in the medium effective in all the soybean explants.
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