PJB-2019-999
RELATIONSHIP BETWEEN ENDOGENOUS HORMONES AND GENDER CONVERSION IN MALE AMUR GRAPE
HAILIN SHEN
Abstract
The amur grape (Vitis amurensis Rupr.) is a dioecious species. In this study, to elucidate the hormonal changes leading to pistil abortion in male amur grapes, we analyzed changes in endogenous hormone content, transcriptional machinery, and gene expression in male amur grapes. The content of endogenous hormones was significantly changed during gender conversion in male amur grape treated with 75 mg·L-1 N1-(2-chloro-4-pyridyl)-N3-phenylurea (CPPU); initially, we observed changes in the contents of zeatin, indole-3-acetic acid, and abscisic acid, followed by changes in gibberellin A3 (GA3) content. The results of Cluster of Orthologous Groups of proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classification showed that hormone signal transduction pathways were closely related to gender conversion in amur grape. By analyzing the expression of eleven differentially expressed genes at eight stages after CPPU treatment, we found that the expression of the indole-3-pyruvate-monooxygenase c25650 was the greatest difference multiple at 3 d after treatment, and the auxin response protein c16214 showed the greatest difference multiple at all other stages after treatment, indicating that auxin synthesis and signal transduction pathways mediated by auxin play a key role in the process of gender conversion in male amur grapes. Altogether, this study provides information regarding the hormonal processes controlling the mechanism of gender conversion in male amur grapes; these findings may lead to further breakthroughs in gender determination studies in Vitis species.
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