PJB-2019-1005
Histopathological changes in Lycopersicon esculentum L. and Solanum melongena L. in response to the infection of Meloidogyne incognita
Shazia Gul
Abstract
Histopathological modifications in Lycopersicon esculentum L. (Tomato) and Solanum melongena L. (Eggplant) in response to root knot nematode (Meloidogyne incognita) infection were studied. For this, 1000 juveniles of M. incognita were inoculated in L. esculentum and S. melongena. Meloidogyne incognita damage was assessed by counting number of galls and number of egg masses on the roots of infected plants. Samples of shoot and root were gathered from the plants and preserved in the formalin-acetic alcohol solution. Anatomical studies of stem, root, midrib and epidermis were done by preparing permanent microscopic slides of preserved sample plants via free-hand sectioning technique and data were collected on various aspects. The recorded data was statistically analyzed and histopathological changes observed were; tissues breakage and various cells with broken walls in cortical region, clustering of gaint cells in stelar region, tissues distortions, elongated nuclei and cavities formation due to the establishment of sedentary females.
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