PJB-2019-1010
PHYTOCHEMICAL EVALUATION AND GENETIC DIVERSITY ASSESSMENT IN ARTEMISIA SPECIES COLLECTED FROM FOOTHILLS OF HIMALAYAS
Aqsa Mir Alam
Abstract
ABSTRACT In the family Asteraceae, Artemisia is a significant genus and has large number of taxa having economic and ecological importance. The aim of study is to analyze highly medicinal products, antioxidant activity and evaluate genetic variability in three species of Artemisia from various sites of district Poonch, located in foothills of great Himalaya. Data was analyzed by using software NTSYS (Numerical Taxonomy and Multivariate Analysis System) pc version 2.01. For the evaluation of phytochemicals and antioxidants different volumes of extracts and solvents were used from leaves and it’s revealed that a large quantity of flavonoids and phenolic contents was present, and the estimation of antioxidant capacity was maximum. The species were also analyzed for genetic diversity within and between various Artemisia species. For molecular investigation, random amplification of polymorphic DNA (RAPD) fragment was utilized to determine genetic variations between and within species. From of ten only six primers gave amplified products. By using all primers total 331 bands were amplified from which 30 were monomorphic, 301 were polymorphic and percentage of polymorphism was 91% in Artemisia japonica, 321 bands were amplified in Artemisia roxberghiana from which 21 were monomorphic, 300 were polymorphic and percentage of polymorphism was 93% as well as 36 bands were amplified in Artemisia absinthium from which 14 were monomorphic, 22 were polymorphic and percentage of polymorphism was 61%. Based on obtained results, it was observed that a significant genetic diversity occurs among the species while little variation was present within the species.
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