PJB-2019-1014
Correlation studies of Ascorbic acid Content and Antioxidant Potential of Asian Produce
Allia Rafique
Abstract
Purpose: Ascorbic acid is one of the potent natural antioxidants. The aim of the present research was to determine and correlate the ascorbic acid content and antioxidant potential of different fresh vegetables and fruits available in Pakistan. Methodology: Ascorbic acid was quantified in various fruits and vegetables by 2, 6-dichlorophenol indophenol (DCPIP) titration method and high-performance liquid chromatography (HPLC) while antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2´-azinobis (3-ethyl-benzothiazoline-6-sulphonate) (ABTS). Standard tests were used for phytochemical screening. Findings: Highest levels of ascorbic acid and antioxidant activity were found in Brassica rapa, Zingiber officinale, Brassica oleracea var. botrytis and Psidium guajava and minimum in Cucumis sativus and Malus domestica. A significant positive correlation was found between level of ascorbic acid and antioxidant potential in aqueous extract of food samples. Practical implications: This work involves evaluation of the quantity of ascorbic acid and antioxidants present in different localised fruits and vegetables which is important due to their extensive protecting role and prevailing demand. Originality: This work involves comparison and correlation of ascorbic acid levels and antioxidant activity of various fruit and vegetables, which is done for the first time for the selected species to the best of our knowledge.
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