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PJB-2019-1017

Phenotypic variation analysis of mutant population of ethyl methane sulfonate in peppers

Huang Dongfu, He Jianwen, Fu Wenting, Hu Mingwen and Yang Hong
DOI:10.30848/PJB2022-1(19)


Abstract

In this study, ethyl methane sulfonate (EMS) was used to chemically mutagenize the seeds of Pepper Zunla-1 to increase the genetic variations of the peppers (Capsicum annuum). The lethal dose 50% (LD50) for peppers was determined by analyzing the relative germination rates of pepper seeds in different concentrations of EMS at different mutagenesis durations, and the mutant phenotypes of 2,271 M1 generation plants and 295 M2 generation plants were investigated. In different developmental stages of the M1 generation, mutations were observed in the leaf shape, floral organ, stem, leaf color, fertility, and fruit shape, and leaf-color and fertility chimeras were also identified. A total of 94 lines in the M2 generation showed mutant phenotypes, with an overall mutation frequency of 31.86%. The types of mutations involved the leaves, stems, fruits, fertility, growth period, and floral organs, accounting for 20%, 34.55%, 21.82%, 7.27%, 12.12%, and 4.24%, respectively, of the overall mutation frequency. Moreover, the mutation types could be further divided into several subtypes. The diverse types of the M2 generation mutants not only were valuable to the applications of genetic modification of peppers, but also had great implications for the discovery of new genes of peppers

To Cite this article: Dongfu, H., H. Jianwen, F. Wenting, H. Mingwen and Y. Hong. 2020. Phenotypic variation analysis of mutant population of ethyl methane sulfonate in peppers. Pak. J. Bot., 54(1): DOI: http://dx.doi.org/10.30848/PJB2022-1(9)
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