PJB-2019-1023
Measurement of Biorational Effect of Imidacloprid on some Aphids spp. as well as on Wheat (Triticum aestivum L.) using Biochemical parameters and ISSR-PCR
Sameer Qari
Abstract
Aphid species including; Rhopalosiphum maidis, Rhopalosiphum padi, Sitobion avenae and Schzaphis graminum are the most common insect pest infesting wheat plants. LC values of imidacloprid against aphid spp. as well as ISSR-PCR molecular markers and biochemical parameters in wheat plant in the present study was carried out, in order to evaluate the mortality % in the aphid also to investigate the effect of imidacloprid on the genetic variation of Triticum aestivum. Aphid spp. reared on wheat seedlings. While, the wheat grains were treated with both the recommended field dose of imidacloprid, and by half such dose (i.e., 2.4 ml/kg and 1.2 ml/kg of seeds), then grown randomly in plant growth chamber in order to investigate the carbohydrate, soluble sugar, protein, free amino acids, DNA and RNA contents and ISSR-PCR analysis. The LC50 of imidacloprid on R. maidis, R. padi, S. avenae and S. graminum were 71.626, 85.853, 91.05 and 115.806 respectively. While, the biochemical parameters in treated wheat plants including; total protein, RNA and DNA contents increased in all experimental groups compared with control. Moreover, total carbohydrates and soluble sugar also increased in treated wheat plants. Also, in treated wheat plants, the genetic diversity studies revealed that among six ISSR primers produced 36 amplified DNA fragments in a molecular weight range of (480-1545 bp.). A 28 fragments were monomorphic, and 8 fragments were polymorphic. It can be concluded that R. maidis was more susceptible to imidacloprid followed by R. padi, S. avenae and S. graminum. Also. the effectiveness of imidacloprid against sucking insects in treated wheat plants may due to few genetic variations and some metabolic changes in the T. aestivum.
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