PJB-2019-1025
ANTI-STRESS PHYTOHORMONES IMPACT ON PROTEOME PROFILE OF GREEN GRAM (VIGNA RADIATA L.) UNDER SALT TOXICITY
Jabeen Farheen
Abstract
Green gram is the chief legume of Pakistan. Thus, current study was conducted to examine the ameliorating effect of phytohormones pre-treatments under salt stress on proteome profile of green gram by SDS-PAGE. The soluble green gram seedling proteins were resolved on 4% stacking and 12% resolving gels. The SDS-PAGE resolved 24 polypeptide bands ranging from 200 to 17 kDa. Among these, 12 out of 24 bands of proteins were essentials house-keeping or growth proteins of green grams. While, 120.0, 114.6, 51.8, 29.1, and 22.8 kDa bands were over-expressed under 50 to 350 mM salt with phytohormones treatments. The other 104.5, 99.8, 95.3, 91.0, 55.0, 46.0, and 17.0 kDa bands were related to the GAᴣ, IAA, and SA induced tolerance. Overall 120, 114.6, 104.5, 99.8, 95.3, 51.8, 29.1, and 22.8 kDa bands were first time identified in this study. Retrieved information from NCBI protein database, the resolved peptides were principally belonging to 7S and 8S vicilin, 2S, 8S, 11S, and 16.5S globulins. It is determined that seed priming with SA enhanced tolerance in green gram by rapidly synthesizing stress alleviating peptides.
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