PJB-2019-1033
SILENCING OF THE SlPIN1 GENE DECREASES THE DROUGHT STRESS TOLERANCE OF TOMATO
TONG PEI
Abstract
The SlPIN gene family is considered to be involved in plant abiotic stress. Our previous studies showed that the relative expression of SlPIN1 under drought stress significantly increased first and then decreased. To further understand the effects of SlPIN1 on tomato plants under drought stress, virus-induced gene silencing (VIGS) was used to downregulate the expression of SlPIN1 in tomato plants. These plants were treated with drought stress, and the resulting changes were analyzed. The efficiency of silencing the SlPIN1 gene was demonstrated by fluorescence quantitative PCR (qRT-PCR) analysis. The MDA content in SlPIN1-silenced plants was significantly higher than that in control tomato plants (CK). The Pro content and SOD activity in the SlPIN1-silenced plants were significantly lower than that in control tomato plants, but the MDA content in the SlPIN1-silenced plants was higher than that in control tomato plants, and the ABA content in the SlPIN1-silenced plants was 38% lower than that in control plants. These results showed that silencing the SlPIN1 gene negatively affects the tomato drought stress response, reducing the tolerance of tomato plants to drought.
To Cite this article:
Download
