PJB-2019-1047
STUDY OF GROWTH RESPONSES IN ZEA MAYS L. GRAINS PRIMED WITH STEM BARK EXTRACTS OF EUCALYPTUS CAMALDULENSIS DEHNH.
Zeb Saddiqe
Abstract
Growth responses in Zea mays L. grains primed with different concentrations of stem bark extracts of Eucalyptus camaldulensis Dehnh. were studied. Stem bark was extracted in methanol (CME) and in n-hexane (HE), dichloromethane (DCME), ethyl acetate (EACE), acetone (ACE) and methanol (ME) sequentially in Soxhlet. Germination was maximum (100%) at 1 and 2 mg/ml concentrations of HE, DCM and ACE and 4 and 8 mg/ml of EACE (92.5% in control group). Minimum germination (87.5%) was at 8 mg/ml concentration of HE and ME. Maximum root length (33.5 cm) and shoot length (28.8 cm) was recorded for DCME at 2 and 1 mg/ml respectively while minimum root length (5.25 cm) and shoot length (1.92 cm) was recorded at 8 mg/ml of CME. Root and shoot length in control group was 24.5 and 26.6 cm. Fresh weight of seedlings was maximum at 2 mg/ml of HE while maximum dry weight was observed at 2 mg/ml of DCME. Except for ACE and ME, all the other extracts were nontoxic for root growth. However, phytotoxic effects of extracts at different concentrations were observed for shoot growth. Maximum seedlings vigor index was at 2 mg/ml of DCME extract and minimum at 8 mg/ml of ME. Maximum tolerance index of root (171.43) was observed for 2 mg/ml concentration of DCME extract while that for shoot (114.4) was observed at 4 mg/ml of HE extract. The results concluded that HE and DCME extracts of E. camaldulensis stem bark can be used for isolation of priming agents.
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