PJB-2019-1054
Antifungal activity of n-butanol stem extract of quinoa against Macrophomina phaseolina
Iqra Haider Khan and Arshad Javaid
Abstract
Quinoa (Chenopodium quinoa Willd.) is a recently introduced pseudo-cereal in Pakistan. The present study aimed to assess the antifungal potential of a polar solvent n-butanol soluble fraction of methanolic stem extract of quinoa against Macrophomina phaseolina. The study was further extended to identify the possible antifungal constituents through GC-MS analysis. In vitro bioassays revealed that a 25 mg mL-1 concentration of the n-butanol fraction in malt extract broth can completely control the fungal growth. GC-MS analysis showed the presence of 9 volatile compounds namely stigmasta-7, 16-dien-3-ol, (3.beta.,5.alpha.)- (23.95%), gamma-sitosterol (16.92%), stigmasterol (13.46%), decane (7.88%), 2-propylcyclohexanol (6.90%), 1,2-benzedicarboxylic acid, diisooctyl ester (5.07%), cyclohexene,3-nonyl- (4.36%), 4-butyl (dimethyl) silyloxpentadecane (3.09%), and 2-pyrrolidinecarboxylic acid-5-oxo-, ethyl ester (1.60%) in this fraction. Literature survey showed that stigmasterol and 1,2-benzedicarboxylic acid, diisooctyl ester had antifungal activities against a variety of fungal species and might be responsible for control of M. phaseolina in the present study
To Cite this article:
Khan, I.H. and A. Javaid. 2022. Antifungal activity of n-butanol stem extract of quinoa against Macrophomina phaseolina. Pak. J. Bot., 54(4): DOI: http://dx.doi.org/10.30848/PJB2022-4(7)
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