PJB-2020-46
Regeneration, callus formation and HPLC determination of some cardiac glycosides of Digitalis lanata Ehrh.
i. Marwa Elsebai Abd El-Sadek
Abstract
In the present study, the seeds of Digitalis lanata Ehrh. were surface sterilized, germinated on hormone-free Murashige and Skoog's culture media supplemented with 30 g/l sucrose and solidified with 5 mg/l Phytagel. Leaves and roots of the obtained 21 days old seedlings were used for callus induction while the leafless dwarf stems and the shoot apical tips were used for regeneration of plantlets. The number of shootlets which could be obtained from single apical shoot tip explant increased from 1.00 ± 0.00 in control group up to 5.00 ± 0.00 with 3 mg/l BA + 3 mg/l Kin treatment and reached up 8.33 ± 0.58 shoots/stem explant in response to the treatment with 3 mg/l BA+ 6 mg/l NAA. Both results are significantly positive increases in comparison to their corresponding controls. Growth curves of calli from both leaf and root explants were plotted over a period of 8 weeks. At the end of growth period, callus from root explants which was compact and yellowish green reached up 7.803 g compared to 3.383 g leaf callus which was friable green. HPLC have shown that callus cultures exceeded the mother plant in biosynthesis of digoxin and digitoxin and that the root callus (445.158 µg/g) was more active than the leaf callus (228.247 µg/g) in comparison to the corresponding control (100 µg/g).Type of explant and growth regulators used affected cardenolides biosynthesis both. The results obtained in this study indicate the promising role of using plant biotechnology in the propagation and secondary metabolite production by D. lanata.
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