PJB-2020-51
Determination of Ankyrin Repeats Domain (ANK) of Rnasel Gene in Hepatitis C Patients and its effects in Viral Load
anum liaquat ali
Abstract
Hepatitis C virus is one of the major globally cause of death and Chronic Hepatitis infection is associated with development of liver cirrhosis and hepatocellular cancer. Different types of gene are also involved as Ankyrin repeat domains of RNASEL gene it perform a significant role in antiviral response. It is regulated by interferon and involved in cleavage of viral RNA. Therefore, aim of this study was to identify Ankyrin repeat domain expression in Hepatitis C positive patients and correlate it with viral load of Hepatitis C. The objectives were to determine expression analyses Ankyrin repeat domains in hepatitis C patients and to correlate HCV Viral load with expressions of Ankyrin repeat domains. Methods: In this study, a total of 80 Hepatitis C RNA positive patient’s whole blood samples were investigated. RNA was extracted from plasma followed by Real Time PCR for quantization of HCV viral load and genotypic analyses. DNA was extracted from these samples followed by PCR amplification of ARK domain. Results: Out of the total 80 patients who were included in study, all had HCV infection. Among them, 48 (63.8%) were males and 32 (36.1%) were females. All patients infected with HCV were found to have genotype 3. Conclusion: We have observed ANK domain in HCV patients’ sample mean range 17.000±15.187. Majority of patients were males and belonged to age group 58-73 years age. The mean age of patients was 50.86 ±14.84 years. All the HCV infected individuals had HCV genotype 3 and had viral loads mean range 837404.21 ±1302318 therefor all high HCV viral load shown Ankyrin repeats domain of RNASE Gene expression mean rang 17.00±15.187.
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