PJB-2020-60
Protoplast isolation and regeneration from leaves and nucellar embryos of Kinnow mandarin (Citrus reticulata Blanco)
Syeda Kahkashan Kazmi, Hammad Afzal Kayani, Marium Raziq, Sheeba Naz, Saifullah Khan and Nurul Kabir
Abstract
Citrus is one of the most dominant horticultural fruit crops in the world. For the genetic improvement of Kinnow mandarin (Citrus reticulate blanco), protoplast isolation and somatic hybridization strategy is suitable. In this study, Kinnow mandarin protoplasts were isolated to get single species regeneration. Protoplasts were obtained from explants like In vitro and field grown leaves and nucellar embryos. Enzymatic protoplast isolation was performed and then yield, and viability testing was done. After initial testing of protoplast their regeneration was achieved. Nucellar embryos gave best regeneration but protoplasts from leaves were unable to regenerate. This study showed that 16 hours incubation was found best for optimum viable protoplasts yield of 5.4 x 104 protoplasts/gm FW (fresh weight) from nucellar embryos and 17.3 x 104 protoplasts/gm FW (fresh weight) of non-viable protoplasts yield from leaves
To Cite this article:
Kazmi, S.K., H.A. Kayani, M. Raziq, S. Naz, S. Khan and N. Kabir. 2022. Protoplast isolation and regeneration from leaves and nucellar embryos of Kinnow mandarin (Citrus reticulata Blanco). Pak. J. Bot., 54(4): DOI: http://dx.doi.org/10.30848/PJB2022-4(27)
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