PJB-2020-67
Effects of Carbon Sources and Synthetic Growth Regulators on the Tissue Culture of Sugarcane
MAZHAR ULLAH
Abstract
Tissue culture protocols with enhanced efficiency are demanded for the effective micro-propagation of sugarcane. Various factors affecting tissue culture must be evaluated and optimize to produce maximum callus and regenerated plantlets subsequently. In this research, different auxins [Dichlorophenoxy Acetic acid (2,4-D), Nephthalene Acetic Acid (NAA)], cytokinin [Benzyl Amino Purine (BAP)] and carbon sources [sucrose, glucose and fructose] with varying concentrations (2, 4 and 6%) were evaluated for their effect on the tissue culture of sugarcane variety-CP 77/400. For callus induction 2,4-D as an auxin was most effective as compared to NAA. BAP and NAA in combination showed high regeneration capacities. Amongst the carbon sources, sucrose was most effective both for callus induction and regeneration. ANOVA revealed significant differences (P ≤ 0.05) amongst the callusing media (CM). Maximum callus induction (48.55%) was achieved on CM-2 augmented with 4 mg L-1 2,4-D and 4% sucrose. All the carbon sources at 4% concentration in CM-2 showed maximum callus induction. However, the best results were shown by sucrose with 50.33% callus induction. Significant differences (P ≤ 0.05) were observed amongst the different shooting media used (SM). Maximum regeneration (72.06%) was observed on SM-2 supplemented with BAP (2 mg L-1), NAA (0.25 mg L-1) and 6% sucrose. Different carbon sources at 6% concentration in SM-2 showed high callus induction however sucrose was more productive and resulted in maximum shoot regeneration (77.98%). Roots were established on ½ MS having 1.5 mg L-1 NAA and regenerated plants were transferred to silt and clay soil (1:1) and were successfully acclimatized to greenhouse condition.
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