PJB-2020-71
Microbiological studies on Rhizobium meliloti isolated from Alfalfa (Medicago Sativa) crop of Killasaifullah District of Balochistan
saqiba jogezai
Abstract
Rhizobium is an important microorganism for the environment because of its nitrogen-fixing ability when in symbiotic relationship with plants (mainly legumes). Rhizobium meliloti symbiosis with plant root hairs and initiates nodule formation in the root inner cortex with subsequent formation of an indeterminate type of nodule. The Rhizobium meliloti are provided shelter inside of the plant's nodules and some of the carbon substrates and micronutrients that they need to generate energy and key metabolites for the cellular processes that sustain life. The present study was designed to evaluate the different microbiological aspects of Rhizobium meliloti isolated from Alfalfa plant. Current research was conducted at “Center for Advanced Studies in Vaccinology and Biotechnology” (CASVAB), University of Balochistan. Total 1000 Alfalfa plant samples were collected from farmer’s field at different locations of Killasaifullah district. The samples were collected from three different (tehsils, Killsaifullah tehsil, Loiband and tehsil Muslim bagh) of Killasaifullah district Total. 80% samples were positive and 20% were negative for Rhizobium meliloti from Alfalfa. The Rhizobium meliloti was found positive for Catalase test and Oxidase test, while negative for Indole production test, Methyl red test, Vogas Proskauer test, and Citrate utilization test. The isolates were also examined for fermentation of the various sugars and result showed that Rhizobium meliloti ferment Glucose, Mannitol, Trehalose, Mannose, Xylose, Cellebiose and Raffinose. The Rhizobium meliloti was confirmed through PCR and the result showed a clear band of 16S rDNA gene with a size of 1500 bp. This study confirmed that the PCR is best way to identify Rhizobium meliloti.
Keywords: Rhizobium, Meliloti, Alfalfa, PCR, Nitrogen
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