PJB-2020-85
MOLECULAR FINGERPRINTING AND ASSESSMENT OF PURITY OF HYBRIDS IN SESAME USING MICROSATELLITE MARKERS
Kanak Saxena
Abstract
Microsatellite markers were used for molecular identification of parental lines and hybrid purity testing in sesame Eighteen microsatellite markers were employed for fingerprinting of eight sesame hybrids and their parental lines. Five SSR markers were found polymorphic across the hybrids and produced unique fingerprint for all the eights sesame hybrids. Further, A set of five polymorphic markers (GBssr-sa-06, GBssr-sa-07, GBssr-sa-08, GBssr-sa-12 and GBssr-sa-184) have been identified as the diagnostic markers differentiated all the hybrids from each other, which can be used as referral markers for unambiguous identification and protection of sesame hybrids. Cluster analysis based on Simple matching (SM) similarity coefficient using UPGMA grouped the parental lines into three clusters. The genetic similarity between the parental lines ranged from 0.43 to 0.83 with an average similarity index of 0.63. A set of diagnostic SSR markers will aid in hybrid breeding programme of sesame.
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