PJB-2020-112
Germination rate and the response of antioxidant enzymes against the salt treatment of Spinacia oleracea (spinach) seedlings
saeeda Bano
Abstract
Introduction: Growing of salt-tolerant crops to meet the global food requirement is crucial due to increase in salinity of agricultural area and several biotic and abiotic factors. Spinach has potential to grow on saline lands. Customarily salt stress caused a reduction in germination rate, however spinach seedlings germinated remarkably well up to 800 mM NaCl concentration and the average weight of seedlings and soluble protein content also increased at high salinity. Methodology: Seed germination was performed at different salt concentrations under-maintained conditions with control. Enzymes extraction was prepared for the response of antioxidant activity at different germination stages of the seedlings. Results and Discussion Results suggested that all antioxidant enzymes play a crucial role during oxidative stress. Under salt stress conditions, the antioxidant enzymes, total protein and germination rate were highly affected. It is suggested that under stress conditions, the antioxidant defense mechanism is activated in the seedling of Spinacia oleracea at very early stage which further helps the plant to survive under harsh conditions.
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