PJB-2020-126
IMPACT OF HEAT-SHOCK AND PHYTOHORMONES APPLICATION ON MORPHO-BIOCHEMICAL TRAITS OF VIGNA RADIATA SEEDLINGS UNDER SALINITY
Dr Jabeen Farheen
Abstract
Vigna radiata L. is the second major pulse of Pakistan. Its yield can be increased by utilizing saline lands. With this respect, the 40 ºC heat-shock was applied as exogenous acclimation treatment, and 100 μM gibberellic acid, 100 μM indole acetic acid and 50 μM salicylic acid was used as seed pre-soaking treatment prior to 0, 50, 150, 250 and 350 mM NaCl stress. The results showed that varieties NM-92, NM-98, NM-51, NM13-1and NM19-19 seedlings length, total fresh biomass, relative water content, and photosynthetic pigments were progressively decreased with 50, 150, 250 and 350 mM salt solution. The highest seedling length (11.33 cm), biomass (4.22 g), RWC (87%), and photosynthetic pigments (1.651 mg gˉ1 FW), related to NM-92 at SA+NaCl 50 mM treatment. While, ascorbate peroxidase (265.33 μM oxidized ascorbate mgˉ1 protein 2 minˉ1), catalase (6.3 μM H2O2 mgˉ1 protein gˉ1FW minˉ1), and superoxide dismutase (24.59 units mgˉ1 protein 30 minˉ1) maximum activities were related to SA+NaCl 350 mM from NM-92, which were significantly differed from HS, GAᴣ, and IAA pre-treatments. In contrast, the lowest malondialdehyde (8.76 μM gˉ1 FW), proline (16.31 μM gˉ1 FW), and hydrogen peroxide (7.01 μM gˉ1 FW) were recorded in SA+NaCl 50 mM treated NM-92 seedlings than other pre-treatments over any varieties. Thus, these results recommended that application of 50 μM SA on mungbean seedlings can be beneficial to grow plants on the highly saline affected zone of the cultivable region.
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