Paper Details

PJB-2020-134

Analysis of genomic regions for crude protein and fractions of protein using a recombinant inbred population in Rice (Oryza sativa L.)

SAJID FIAZ
Abstract


The improvement of seed storage proteins (SSPs) is integral for rice breeding of superior nutritional quality. To understand the genetic architecture of SSP content and to unearth novel alleles will be valuable to develop elite cultivars. A total of 44 QTLs with relation to PC, GLU, GLO, ALB and PRO were detected on all 12 chromosomes, with single QTL explaining 3.70-12.47 % of phenotypic variation. The majority of detected QTLs located in the region of RM7158-RM3414 which includes Waxy (Wx) gene along with qPC1, the major QTLs affecting contents of protein and different protein fractions. The fractions of protein exhibited highly positive significant correlation with each other indicating that these traits are related to each other. Three QTLs (qPC6, qPC7 and qGLU6) had shown phenotypic contribution rate relatively higher, suggesting that the QTLs expression of PC and GLU were influenced by the environmental conditions. Whereas, a pair ofepistatic QTL, 1 for GLO and 1 for PRO were also detected meanwhile, the M-QTLs were the primary genetic basis for PC, GLU and ALB, respectively. The present study results, demonstrated that a QTL located on chromosome 6 which might be located within the Wx gene region influencing protein and fractions of protein. The application of present study results will facilitate further understanding for the genetic foundation of protein and fractions of protein and can be utilized for future grain nutritional quality improvement programs.

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