Online Submission



Register | Forgot password?

Recent Issues

Uploaded: 01/06/2021 Uploaded: 02/04/2021
Uploaded: 09/03/2021 Uploaded: 09/03/2021

Latest News
Thursday, March 30, 2017

ISI Impact Factor 0.972

 

Details
Paper Details

PJB-2020-153

Molecular phylogeny of different species of family Verbenaceae using chloroplast rps14 gene

Ayesha Malik, Shahana Arif, Wasim Akhtar and Tariq Mahmood
DOI:10.30848/PJB2022-1(10)


Abstract

Family Verbenaceae  has immense importance for ornamental, forage resources, aromatic and medicinal purposes. In the present study chloroplast rps14 gene is used for determination of phylogenetic relationship among Verbenaceae   species and structural validation of rps14 protein through Ramachandran plots. The rps14 gene was amplified and sequenced analysis was done by MEGA7, I-TASSER and RAMPAGE. In phylogram the Verbenaceae   species show little genetic distance of 0.0050 that revealed close genetic relationship between them. The genetic diversity ranges from 0.092 to 0.017 and value of overall mean distance was 0.067. The nucleotide diversity in Tajima’s Neutrality Test was 0.063165. The phylogenetic tree based on rps14 gene depicted close relationship of Verbena tenuisecta, V. officinalis, V. bonariensis and V. incisa among them with BS value of 67% The Duranta erecta and Citharexylum spinosum show close relationship in group I with BS value of 57%. In Group II V. tenuisecta and V. officinalis depicted close phylogenetic association with BS value of 100%. The rps14 protein structure validation by RAMPAGE revealed that V. tenuisecta, C. spinosum, V. bonariensis, V. incisa and P. volubilis had good quality protein structural models because these species had ≤ 2% amino acid residues occurred in the outlier region. This study shows close genetic relationship and low genetic diversity between Verbenaceae   species. The low nucleotide diversity also revealed close relationship between Verbenaceae   species. The phylogenetic tree indicated close genetic relationship between Verbenaceae   species with well supported BS values. The validation of rps14 protein structure by RAMPAGE predicted the best quality protein structural models. This study demonstrated the rps14 gene as useful marker for assessment of phylogenetic studies.

To Cite this article: Malik, A., S. Arif, W. Akhtar and T. Mahmood. 2022. Molecular phylogeny of different species of family Verbenaceae using chloroplast rps14 gene. Pak. J. Bot., 54(1): DOI: http://dx.doi.org/10.30848/PJB2022-1(10)
Download

About Journal
--------------------------------------
Author's Guideline
--------------------------------------
Submit Online
--------------------------------------
In Press Articles
--------------------------------------
Status of Manuscript
--------------------------------------
Current Impact Factor 0.972
--------------------------------------
5 Years Impact Factor 0.85
Indexing

PJB is indexed in following international databases: View all

123123123123


Thomson Reuters ISI Web of Knowledge


SCOPUS

https://www.scopus.com/sourceid/19983?origin=sbrowse


AGRIS


ASCI-Database


CABI


International Scientific Indexing (ISI)


Cambridge Scientific Abstract


Chemical Abstract Services


E-Journals


ISC (Islamic World Science Citation Center)


Scientific Indexing Services


SCIMAGO


Index Copernicus (IC)


ProQuest


DOAJ


Google Scholar


Contact Us
Pakistan Journal of Botany, Botanical Garden, University of Karachi, Karachi, Pakistan

pakjbot@pakbs.org | Facebook
Hit Counter: 3233279, Today's: 733, Yesterday's: 1530, Country Wise Counter

Copyright © 2017-18 - All Rights Reserved - www.pakbs.org