PJB-2020-162
Effect of introducing a Trichoderma harzianum chitinase gene to tuber mustard against white mold
Seyedmohammadreza Ojaghian
Abstract
This study was carried out to evaluate the effect of introducing chit42 to tuber mustard plants through Agrobacterium-mediated transformation against white mold caused by S. sclerotiorum. The binary plasmid pGisPEC1 was used in this study. PCR analysis detected the transgene in 27 transformants with a transformation efficiency of 6.9 %. Southern blot test was used to assess the copy number of transgene in tuber mustard plants. One, two, two and two chit42-related bands were observed in the transformed lines TMB4, TMB7, TMB12 and TMB18, respectively. Enzymatic tests showed a significant increase in the activity of endochitinase in protein extracted from leaf tissues of chit42 transgenic 75-day tuber mustard lines. The pathogenicity of three pathogen isolates was tested on the leaves of transformed plans. The results revealed that introducing the gene chit42 to tuber mustard plants markedly reduced infection radius on the leaves seven days after inoculation with the pathogen.
To Cite this article:
Download
