PJB-2020-174
Nitric oxide (NO) involved in Cd tolerance in NHX1 transgenic duckweed during Cd stress
Na Li
Abstract
Anthropogenic activities cause heavy metal pollution such as cadmium (Cd). Na+/H+ Antiporter (NHX1) has been proved to enhance salt tolerance. In our previous study, NHX1 transgenic duckweed showed cadmium tolerance, and the signal mechanism needs to be explored. Nitric oxide (NO) is an important signaling molecule involved in the response to abiotic stress. This study analyzed the levels of endogenous NO in wild-type (WT) and NHX1 under cadmium stress. 24 h Cd treatment reduced the level of NO signal in WT duckweed, and the NO level in NHX1 duckweed was significantly higher than that in WT. Studies have proved that NHX1 influence pH. The level of NO in this study has been investigated at different pH. The NO level was the highest in the duckweed cultured at pH5.3. Nitrate reductase gene expression was down-regulated and NO synthesis was decreased under cadmium stress.
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