PJB-2020-196
Genetic Diversity and Population Structure Analysis of 10 Species of Ranunculaceae by ISSR Markers
Yonghui Li
Abstract
Objective: To analyze genetic diversity in 10 species of Ranunculaceae. Methods: The genetic diversity and genetic structure of 10 species of Ranunculaceae in 22 populations in Luoyang and surrounding areas were analyzed using primers selected by ISSR molecular markers. Results: The 9 selected primers amplified a total of 116 clear bands, and the proportion of polymorphic bands was 100%. The average polymorphism information content (PIC) of the primers was 0.9478. The results of genetic diversity analysis showed that the Shannon information index (I) of 22 populations of Ranunculaceae plants was 0.4367 0.1904, and Nei's genetic diversity index (H) was 0.2807 0.1481. The above results showed good polymorphism in all 9 primers, and very rich genetic diversity in the 10 species of Ranunculaceae from 22 populations. The gene flow Nm was 0.3096 and genetic differentiation index Gst was 0.5997, indicating that genetic differentiation mainly derived from among populations, with less gene communication among populations. The Mantel test showed positive correlation between genetic distance and geographical distance (r = 0.2530, P < 0.01). Cluster analysis, principle coordination analysis(PCoA) and population cluster analysis yielded broadly consistent clustering results showing that individuals of the same germplasm were closely related, tending to be clustered into one group first; the second grouping was arranged according to the geographical distance. Conclusion: The genetic diversity of 10 species of Ranunculaceae in 22 populations is very rich, and interspecific differentiation is the main reason for the genetic diversity; However, this does not rule out the possibility that external factors (such as the environment) will have also affected diversity. This study lays the foundation for rational utilization and resource management of Ranunculaceae.
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