PJB-2020-198
Characterization of Nano-Silver Phytosynthesized from Mentha longifolia L. and Evaluation of their Photodynamic Potential
BILAL JAVED
Abstract
The present study deals with the ecofriendly one-pot synthesis and stabilization of silver nanoparticles (AgNPs) by using aqueous extract of Mentha longifolia branches. Spectrophotometric analysis of different ratios of reactants revealed that a 1 to 9 ratio of plant extract and silver salt solution respectively is the most suitable proportion for synthesis. Synthesis of AgNPs was confirmed by the observation of change in color of the reaction mixture which was carried out at 60 °C by using 3 mM of silver salt and pH of the reaction medium was maintained at 5.22. A characteristic surface plasmon resonance (SPR) band was observed at 495 nm of light wavelength. SEM images revealed that the nanoparticles are in ~20-80 nm and are anisotropic and nearly spherical while EDX analysis showed the presence of elemental Ag with ~90% signal intensity. Size distribution analysis of the AgNPs was performed by dynamic light scattering technique and AgNPs were found in the range of ~8-30 nm. ROS quantification revealed that the AgNPs have a quantum yield of 0.09 Φ which provides them the ability to proteolytically treat cancer and other microbial pathogenic cells. AgNPs did not report any photothermal activity to be used as photodynamic agents. These findings explain the oxidative potential of M. longifolia to bio-fabricate AgNPs and their abilities to generate ROS may help to curb dreading diseases.
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