PJB-2020-220
ANATOMICAL TOOL IN THE IDENTIFICATION OF PLOIDS IN MAIZE SEEDLINGS AND POTENTIAL USE IN INITIAL STAGE OF DOUBLE HAPLOIDS OBTAINMENT PROCESS
Raquel Maria de Oliveira Pires
Abstract
Studies that optimize the haploid technique in the removal of maize (Zea mays L.) lines are necessary. Morphological markers are commonly used but have strong influence of the environment, and laboratory methods have been developed and may be more efficient. The objective was to study the use of anatomical analysis tool in young maize leaf for use as indirect markers in the identification of ploidys. Hybrids were crossed with the KEMS haploid inducer. The seeds crossed, were selected according to the R-navajo marker and submitted to two different protocols of chromosome duplication. After the self-polinization of the DH0 plants, the DH1 seeds were taken to the field, divided into treatments according to the parentals and duplication protocols. Leaf tissue samples were collected to the evaluation of the amount of DNA and identification of ploidys and anatomical analysis at V4 stage. The nuclear DNA review of each sample was performed for the comparison in histograms of the position of G1 peak to the G1 peak of the internal or external reference standard. A high accuracy came to validate an anatomical tool, through the variables studied in this work, as a marker in the differentiation of ploidis in maize plants, and it can be used in selection programs. The anatomy tool, together with the flow cytometry technique, can be used as an indirect method in haploid cutting programs at the initial stage of the identification of seedlings.
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