Online Submission

Register | Forgot password?

Recent Issues

Uploaded: 01/06/2021 Uploaded: 02/04/2021
Uploaded: 09/03/2021 Uploaded: 09/03/2021

Latest News
Thursday, March 30, 2017

ISI Impact Factor 0.972


Paper Details


Evaluation of cytotoxicity and genotoxicity of treated wastewater in Almadinah Almonawrah, Saudi Arabia using Allium cepa assay

Mohammad K. Alotaibi


The aim of this study was to evaluate the cytotoxicity and genotoxicity of treated wastewater that is discharged in the downstream Alhamed valley, in Almadinah Almonawrah city, Saudi Arabia. Treated wastewater samples were collected from the outlet of sewage treatment plant (STP) and three downstream locations, approximately (10, 20, and 30 km) of the plant. The cytotoxicity and genotoxicity of the samples were assessed using Allium cepa mitotic index (MI) and chromosome aberrations (CA) assays respectively. Onion bulbs were exposed to different concentrations (100, 50, 25, and 12.5%) of treated wastewater for 12 hours. Bulbs exposed to tap water were used as negative controls. Chromosome aberrations (CA) were induced by treated wastewater in a dose dependent manner. In addition, CA were significantly higher at the outlet of STP than the downstream locations. Moreover, the frequency of CA decreased as the distance from STP increased. With respect to cytotoxicity, treated wastewater from all locations induced significant decreases in MI and significant increases in the frequency of aberrant cells except the 30 km sampling point (concentration 12.5%). In conclusion, treated wastewater seems to possess genotoxic and cytotoxic compounds that get eliminated by movement of the water away from STP.

To Cite this article: Alotaibi, M.K. 2022. Evaluation of cytotoxicity and genotoxicity of treated wastewater in Almadinah Almonawrah, Saudi Arabia using Allium cepa assay. Pak. J. Bot., 54(1): DOI:

About Journal
Author's Guideline
Submit Online
In Press Articles
Status of Manuscript
Current Impact Factor 0.972
5 Years Impact Factor 0.85

PJB is indexed in following international databases: View all


Thomson Reuters ISI Web of Knowledge





International Scientific Indexing (ISI)

Cambridge Scientific Abstract

Chemical Abstract Services


ISC (Islamic World Science Citation Center)

Scientific Indexing Services


Index Copernicus (IC)



Google Scholar

Contact Us
Pakistan Journal of Botany, Botanical Garden, University of Karachi, Karachi, Pakistan | Facebook
Hit Counter: 3232417, Today's: 1401, Yesterday's: 1916, Country Wise Counter

Copyright © 2017-18 - All Rights Reserved -