PJB-2020-247
Alkaline pre-treatment of wheat straw for production of ethanol using Saccharomyces cerevisiae SS-4 immobilized in nanoparticles
Sidra Sarwar, Arifa Tahir, Ikram Ul Haq and Faiza Anum
Abstract
This study was designed to evaluate cost effective bioprocess to enhance production of bioethanol by utilizing immobilized S. Cerevisiae SS-4 in calcium alginate (Ca) beads and calcium alginate magnetic nanoparticles (Ca-MNP) beads to improve bio ethanol production using wheat straw.The method of pre-treatment had a pronounced effect on the yield of reducing sugars.Maximum delignification (58%) and 36.31% saccharification was observed with 2.5% (v/v) NaOH. As concentration of NaOH increases, cellulose content decreases while increase in weight loss was observed. Results showed that immobilized cells of S. cerevisiae SS-4 in Ca-MNP gave high yield of ethanol (49.71g/L) as compared to immobilized cells of S. cerevisiae SS-4 in Ca (45.66 g/L) and free cells (36.52g/L) at pH 4.5, 28 ºC for 72 hrs. Magnet recovery method was used to recover nanoparticles and reusability was evaluated after five time usage
To Cite this article:
Sarwar, S., A. Tahir, I.U. Haq and F. Anum. 2022. Alkaline pre-treatment of wheat straw for production of ethanol using Saccharomyces cerevisiae SS-4 immobilized in nanoparticles. Pak. J. Bot., 54(1): DOI: http://dx.doi.org/10.30848/PJB2022-1(45)
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