PJB-2020-1538
In-silico transcriptome study of the rice (Oryza sativa) strigolactone-deficient (dwarf17) mutant reveals a potential link of strigolactones with various stress-associated pathways
Fahad Nasir, Lei Tian, Ji Li, Shasha Luo, Asfa Batool, Ali Bahadur, Weiqian Li and Chunjie Tian
Abstract
Strigolactones (SLs), the newest group of phytohormones, are involved in a wide range of functions, including the regulation of plant growth and physiology. Besides, emerging evidence suggests that SLs also participate in the promotion of plant environmental stress resilience through mediation of different metabolic genes/pathways. However, thus far little is known about SL-mediated transcriptional changes in rice (Oryza sativa), compared to other model plants. To meet this objective, we analyzed the RNA-seq-based comparative transcriptome data sets of rice SL-deficient dwarf17 (d17) mutant line and its respective wild-type (WT), obtained from the National Center for Biotechnology Information GenBank. Both, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, in order to identify genes/pathways which function downstream of SLs. With respect to the WT, a large number of functional GO terms, mainly stress-associated terms such as ‘response to stress’, ‘response to stimulus’, ‘response to chemical’, ‘response to oxidative stress’ and ‘reactive oxygen species metabolic process’ were significantly suppressed in the d17 mutant plants. In addition, KEGG metabolic pathways such as ‘valine, leucine and isoleucine degradation’, ‘plant hormone signal transduction’, ‘galactose metabolism’, ‘fatty acid degradation’ and ‘phenylalanine metabolism’ were also remarkably undermined in the d17 lines relative to the WT. These results imply a possible involvement of rice SLs in the regulation of distinct stress-related metabolic genes/pathways, which may function in environmental stress tolerance of plants. Taken together, the study provides new opportunities to broaden our limited understanding of SL-regulated downstream pathways, especially in rice
To Cite this article:
Nasir, F., L. Tian, J. Li, S. Luo, A. Batool, A. Bahadur, W. Li and C. Tian. 2020. In-silico transcriptome study of the rice (Oryza sativa) strigolactone-deficient (dwarf17) mutant reveals a potential link of strigolactones with various stress-associated pathways. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(6)
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