PJB-2020-423
Morphology and phylogeny of important medicinal plant Berberis lycium Royle based on matK, rbcL, ITS and trnH-psbA from Azad Jammu and Kashmir
Syeda Maria Fiaz Bukhari, Ghazanfar Ali, Zeeshan Anjum, Tasleem Akhtar, Wasim Akhtar and Syed Rizwan Abbas
Abstract
Berberis lyceum Royle has a long history of medicinal uses to treat different diseases. Dry fruits and roots of this species are medicinally important and are extensively used in many parts of the world. The samples of this species were randomly collected from five districts of Azad Kashmir, Pakistan, including 35 locations. In this study fruits, leaves, stem, roots, and thorn of B. lyceum were used. The morphological studies were conducted to evaluate its qualitative and quantitative traits. For genomic analysis, DNA was extracted from fresh leaves and confirmed on 1 % agarose gel electrophoresis. Fifteen samples were selected for phylogenetic analysis by using four markers including matK, rbcL, ITS, and trnH-psbA. Morphological data showed a difference in their values due to the variations in their altitude, climatic conditions, and soil texture. The phylogenetic study and sequence demarcation tool (SDT) analysis revealed that B. lycium Royle sequences identified in the current study are genetically very similar to each other and they developed the distinct clade with very close isolates previously reported and their pairwise sequence identity (PSI) score is more than 99% among themselves. All the genetic markers (matK, rbcL, ITS and trnH-psbA) successfully clustered the sequences and revealed that these markers can be used for species authentication of B. lycium. The 3D protein structural models for matK protein sequences were predicted through I-TASSER. Models having the highest C-score were selected for Ramachandran plot analysis and indicated that 3D protein models of selected samples of B. lyceum were satisfactory. The findings of the current study are very important for the future identification and conservation of this medically important species in the region
To Cite this article:
Bukhari, S.M.F., G. Ali, Z. Anjum, T. Akhtar, W. Akhtar and S.R. Abbas. 2022. Morphology and phylogeny of important medicinal plant Berberis lycium Royle based on matK, rbcL, ITS and trnH-psbA from Azad Jammu and Kashmir. Pak. J. Bot., 54(2): DOI: http://dx.doi.org/10.30848/PJB2022-2(39)
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