PJB-2020-506
Isolation and identification of novel leaf spot pathogen Fusarium equiseti from Spinacia oleracea
Shazia Shafique, Ayesha Mubarak, Sobiya Shafique, Abrar Hussain, Alina Javed and Rubab Rafique
Abstract
In recent past spinach is threatened by various fungal, viral and bacterial diseases which are major cause of loss in crop production. Diseases caused by fungal pathogen, rank amongst the highest cause of spinach yield loss. During the field survey of selected areas of Punjab, spinach samples infected with leaf spot were collected and disease severity was recorded. The fungal pathogen was isolated from diseased spinach samples and based on morphological characterization and analysis of rDNA region of internal transcribed spacer (ITS) sequence, partial elongation factor (EF1/EF2) and Beta tubulin (Bt2a/Bt2b), the isolate was identified as Fusarium equiseti var. bullatum (Sherb). of Spinacia oleraceae. The pathogenicity trails conducted in plates as well as in pots confirmed Fusarium equiseti, as the most virulent pathogen of spinach. To our knowledge, this is the first report of isolation of a novel pathogen from S. oleraceae.
To Cite this article:
Shafique, S., A. Mubarak, S. Shafique, A. Hussain, A. Javed and R. Rafique. 2022. Isolation and identification of novel leaf spot pathogen Fusarium equiseti from Spinacia oleracea. Pak. J. Bot., 54(3): DOI: http://dx.doi.org/10.30848/PJB2022-3(21)
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