PJB-2021-1523
Comparative metabolomics reveals the inhibition of glutamine and pyruvic acid synthesis closely related to pepper cytoplasmic male sterility
Chai Weiguo, Fang Xianping, Xiao Wenfei, Xin Ya, Liu Xinhua and Qiu Jieren
Abstract
To investigate the mechanisms of cytoplasmic male sterility (CMS) in pepper (Capsicum annuum L.), cytological observation and metabolomics analysis of different pollen development processes were carried out. The pollen development processes were divided into five stages. Resin semi-thin section analysis indicated an abnormality of the sterile anthers started at the tetrad stage (S2); the cell of the tapetal layer was over-vacuolated. The tapetal began to degrade and lose its function. The tetrad could not get essential energy and materials which resulted in pollen abortion. Anthers of S2, and uninucleate pollen stage (S3), were selected and analyzed by non-target metabolomics. A total of 454 metabolites in S2 and S3 stages of maintainer line were detected, and 201 were annotated. For the CMS line, 459 metabolites in two stages were detected and 211 were annotated. Alanine, arginine, serine, and tryptophan were found in the two stages of maintainer line. A marked decrease of glutamine and pyruvic acid during the pollen abortion process were especially found in the pepper CMS line. The decrease of pyruvate concentration further leads to the abnormal synthesis of various kinds of amino acids and ATP which may ultimately contribute to the phenotype of the CMS line.
To Cite this article:
Weiguo, C., F. Xianping, X. Wenfei, X. Ya, L. Xinhua and Q. Jieren. 2021. Comparative metabolomics reveals the inhibition of glutamine and pyruvic acid synthesis closely related to pepper cytoplasmic male sterility. Pak. J. Bot., 53(3): DOI: http://dx.doi.org/10.30848/PJB2021-3(11)
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