PJB-2006-86
CHARACTERIZATION AND DOCUMENTATION OF BACTERIAL DIVERSITY COLLECTED FROM VARIOUS LOCAL HABITATS-1. DIVERSITY IN ESCHERICHIA COLI
RUBINA ARSHAD*, SHAFQAT FAROOQ AND SYED SHAHID ALI
Abstract
Escherichia coli strains have been collected from different local habitats including soil, water, plants, animals and human faces. These strains were characterized for different penicillin acylases (PA), which hydrolyse penicillin G and/or V to yield 6-aminopenicillanic acid (6-APA): a key intermediate of semi synthetic penicillin. The study was conducted with the objectives to describe i) parameters used to differentiate different isolates, ii) to report variation in strains to produce various acylases, iii) to describe the importance and utilization of various strains and iv) to introduce to other microbiologists the E. coli strains collected and maintained by bacterial diversity collection section (BDCS) of NIAB. Our ultimate aim is to maintain a collection of locally isolated E. coli to supplement the on-going efforts of isolating new PA producing bacteria. About 98 % of the collected E. coli strains can produce penicillin G and amplicillin acylases while penicillin V acylase was exhibited by 57% strains. Co-existence of penicillin acylase and -lactamase was observed in all the strains. Some of the strains also possessed co-existence of more than three acylases. Based on the variability in the production of various acylases, 79 E. coli strains were differentiated, which are being described with the hope that in the post WTO era these strains would be able to provide the help to local industry and researchers.
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